| 13/03/10 | Cisco Roma | Aversa Normanna | 3 – 0 | (2 – 0) |
| 14/03/10 | Scafatese | Vico Equense | 0 – 0 | (0 – 0) |
| Siracusa | Monopoli | 0 – 0 | (0 – 0) | |
| Noicattaro | Barletta | 1 – 1 | (0 – 1) | |
| Melfi | Gela | 2 – 2 | (2 – 0) | |
| Cassino | Igea Virtus | 2 – 1 | (1 – 0) | |
| Brindisi | Juve Stabia | 1 – 2 | (1 – 1) | |
| Vibonese | Manfredonia | 3 – 0 | (2 – 0) | |
| Catanzaro | Isola Liri | 3 – 0 | (1 – 0) |
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