| 17/01/10 | San Marino Calcio | Gubbio | 0 – 1 | (0 – 0) |
| Sangiovannese | Nocerina | 0 – 2 | (0 – 1) | |
| Pro Vasto | Prato | 2 – 0 | (1 – 0) | |
| Poggibonsi | Sacilese | 1 – 1 | (0 – 0) | |
| Colligiana | Bassano | 1 – 1 | (1 – 1) | |
| Lucchese | Bellaria Igea | 4 – 1 | (1 – 1) | |
| Sangiustese | Celano O. | 2 – 1 | (0 – 1) | |
| Giacomense | Itala San Marco | 1 – 1 | (0 – 0) | |
| Fano Alma | Carrarese | 3 – 1 | (0 – 1) |