| 13/12/09 | Sacilese | Celano O. | 1 – 2 | (0 – 1) |
| Lucchese | Sangiustese | 3 – 0 | (1 – 0) | |
| Bellaria Igea | Giacomense | 2 – 1 | (0 – 0) | |
| Gubbio | Poggibonsi | 1 – 1 | (1 – 0) | |
| Sangiovannese | San Marino Calcio | 2 – 2 | (1 – 1) | |
| Nocerina | Fano Alma | 1 – 1 | (0 – 0) | |
| Colligiana | Pro Vasto | 1 – 1 | (0 – 1) | |
| Bassano | Carrarese | 2 – 0 | (0 – 0) | |
| Itala San Marco | Prato | 0 – 2 | (0 – 0) |